IFATS Recommendations for FDA Regulation of Human Cells, Tissues, and Cellular and Tissue-Based Products
International Federation of Adipose Therapeutics and Sciences (IFATS)
45 Lyme Road – Suite 304
Hanover, NH 03755 USA
Tel: 1-603-643-2325, Fax: 1-603-643-1444
September 26, 2016
Division of Dockets Management (HFA–305) Food and Drug Administration
5630 Fishers Lane, Rm. 1061
Rockville, MD 20852
Re: FDA-2014-D-1856 – Comments to 2014-2015 Draft Guidance regarding:
- Docket FDA-2014-D-1584: “Same Surgical Procedure Exception under 21 CFR 1271.15(b): Questions and Answers Regarding the Scope of the Exception; Draft Guidance for Industry”;
- Docket FDA-2014-D-1696: “Minimal Manipulation of Human Cells, Tissues, and Cellular and Tissue-Based Products; Draft Guidance for Industry and Food and Drug Administration Staff”;
- Docket FDA-2014-D-1856: “Human Cells, Tissues, and Cellular and Tissue-Based Products from Adipose Tissue: Regulatory Considerations; Draft Guidance for Industry”;
- Docket FDA-2015-D-3581: “Homologous Use of Human Cells, Tissues, and Cellular and Tissue- Based Products; Draft Guidance for Industry and FDA Staff.”
Dear Sirs and Madams:
The International Federation of Adipose Therapeutics and Sciences (IFATS) appreciates this opportunity to submit the following comments to supplement its earlier written comments and recent testimony at the September 12-13, 2016 Public Hearing on the 2014-2015 Draft HCT/P Guidances concerning: a) Minimal Manipulation; b) Same Surgical Procedure; c) Adipose Tissue; and d) Homologous Use.
IFATS is committed to the responsible advance of the science and translation of new adipose therapies, and it is determined to ensure patient safety. It was founded in 2003 by pioneering adipose stem cell biologists and clinician–scientists with the goal of advancing the science of adipose tissue biology and its clinical translation to therapeutic applications. Since that time, IFATS has remained at the forefront of regenerative medical applications involving adipose tissue and cells. Membership now spans 40 countries in North America, Europe, Africa, the Middle East, Asia, Australia, and Central and South America, and includes basic scientists, translational researchers, clinicians, and regulatory and biotech representatives. IFATS is formally aligned with, and its members serve on the editorial boards of the prestigious journals, Stem Cells and Stem Cells Translational Medicine. With the International Society for Cellular Therapy (ISCT), IFATS has provided the scientific community with a detailed description and definition of adipose derived cells (both stromal vascular fraction, or SVF, and adipose-derived stromal/stem cells, or ASCs) in the formal publication entitled Cytotherapy. Thus, IFATS possesses the necessary expertise to assist regulatory agencies in understanding adipose tissue, and regulating the safety and efficacy of adipose-related products and therapies.
Drawing on this expertise, IFATS has reviewed the 4 draft guidances with great care. It respectfully requests the FDA to reconsider and modify the 4 draft HCT/P guidances as follows:
Recommendation #1 – Cell-Based Risks: Interpret and evaluate an HCT/P’s homologous use and minimal manipulation based on its manufacturer’s intended use in the patient.
Recommendation #2 – Provider-Based Risks: Reduce provider-created risks by targeting provider behavior.
Recommendation #3: Recognize that adipose HCT/Ps have both structural and nonstructural functions, and regulate based on its manufacturer’s intended use in the patient.
Recommendation #4: Revise the evaluation of minimal manipulation and homologous use as they pertain to particular applications of adipose tissue.
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IFATS recognizes the FDA’s challenge in developing regulations that fulfill the agency’s dual and interrelated responsibilities of protecting patients while promoting innovation. IFATS further recognizes that although these are complementary rather than competing objectives, they are often difficult to pursue simultaneously. The FDA’s 3-tiered, risk-based §§ 361 – 351 framework balances these concerns by making the degree of regulatory oversight proportionate to the degree of an HCT/P therapy’s risk.
The concepts of homologous use and minimal manipulation are key determinants of whether an HCT/P will be classified as a § 361 product (which does not need premarket approval) or a § 351 drug, device and/or biological product (which requires formal premarket approval). The applicability of § 351’s “same surgical procedure” exception also turns on homologous use and minimal manipulation. For most manufacturer-clinicians, § 351 categorization raises insurmountable obstacles due to the time and expense of obtaining premarket approval. In such cases, § 351 classification effectively prohibits access to safe and effective HCT/P therapies, even when those therapies involve a patient’s own cells and/or can deliver superior results with reduced risks. At the same time, § 351 oversight is essential for therapies that pose greater risks due the HCT/P’s characteristics, mechanism(s) of action and circumstances of use.
A second type of risk involves rogue clinicians offering false promise in the form of unproven therapies performed with few safeguards and less training. Provider misconduct is not unique to HCT/P therapies; it pervades all areas of medical practice. Nevertheless, IFATS shares the FDA’s alarm over such practices in the context of HCT/Ps, and is equally determined to curtail them. Because a solution cannot solve a problem without identifying and attacking its root cause, effective regulation of HCT/P-related risks must recognize and respond to their multivariate causes. Put simply:
- Sections 351 and 361 appropriately attempt to regulate HCT/P therapies proportionate to the risks of unpredictable and/or unsafe cell behavior.
- However, the risks of untrained providers misusing HCT/P therapies are caused by providers misbehaving, not cells misbehaving.
Consequently, interpretive guidance that restricts the definition and application of HCT/P terminology can only go so far in restricting provider-based risks. In addition, restrictive, inaccurate or imprecise definitions and interpretations carry their own risks of restricting access to therapies and restricting a patient’s right to evaluate risk through the process of informed consent.
Therefore, IFATS recommends that the FDA adopt an overall two-part strategy that focuses on both categories of HCT/P risks, i.e., those relating to cell behavior and those that pertain to provider behavior.
Recommendation #1 – Cell-Based Risks:
Interpret and evaluate an HCT/P’s homologous use and minimal manipulation based on its manufacturer’s intended use in the patient. Interpretive guidance should predicate each definition on to the functions and/or characteristics of the specific composition (i.e., cell type(s) and/or matrix or other component(s)) that are involved in, and/or relevant to the manufacturer- clinician’s intended use in the patient.
Recommendation #2 – Provider-Based Risks:
To reduce provider-created risks, the FDA should target provider behavior by collaborating with IFATS and comparable organizations to draw on and supplement existing federal and state methods of certification, registration, and similar measures.
Adopting this two-part strategy can control risk more comprehensively – and therefore more effectively – in furtherance of the FDA’s dual and interrelated obligations of protecting patients and promoting the availability of HCT/P therapies. IFATS explains each recommendation as follows:
Recommendation #1 – Cell-Based Risks: Interpret and evaluate an HCT/P’s homologous use and minimal manipulation based on its manufacturer’s intended use in the patient.
The four draft guidances on homologous use, minimal manipulation, same surgical procedure and adipose tissue individually and collectively intend to “improve stakeholders’ understanding” of 21 CFR 1271 by clarifying the FDA’s interpretation of homologous use and minimal manipulation. As demonstrated by the initial round of public comments and the ensuing public hearing on September 12 and 13, 2016, the draft guidance documents have not clarified applicable regulations. They have instead compounded the difficulty of understanding and complying with them. The drafts’ introduction of new definitional inaccuracies has also amplified rather than reduce patient risk.
IFATS respectfully requests the agency to clarify the definitions and application of homologous use and minimal manipulation by interpreting each as referring to the characteristics of the specific cell type(s) and/or the matrix or other component(s) that are involved in, and/or relevant to the manufacturer’s intended use in the patient. Thus, the definition of homologous use with interpretive guidance would read as follows:
21 CFR 1271.3(c): Homologous use means the repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues with an HCT/P that performs the same basic function or functions in the recipient as in the donor.
Recommended GUIDANCE: As used in this section, “performs the same basic function or functions in the recipient as in the donor” shall be interpreted as referring to one or more of the function(s) of the specific composition of the therapeutic/product, reflecting the specific cell type(s) and/or the specific matrix or other component(s) in the donor tissue that are involved in, and/or relevant to the manufacturer’s intended use in the patient.
Similarly, the definition of minimal manipulation with interpretive guidance would read as follows:
21 CFR 1271.3(f) Minimal manipulation means:
- For structural tissue, processing that does not alter the original relevant characteristics of the tissue relating to the tissue’s utility for reconstruction, repair, or replacement;
- For cells or nonstructural tissues, processing that does not alter the relevant biological characteristics of cells or
Recommended GUIDANCE: As used in this section, “relevant” characteristics shall be interpreted to mean the characteristics of the specific cell type(s) and/or the specific matrix or other component(s) in the donor tissue that are involved in, and/or relevant to the manufacturer’s intended use in the patient.
Rationale: Incorporating and relying on the manufacturer’s intended use harmonizes the interpretation and definition of homologous use and minimal manipulation with statutory directives to predicate the regulation of drugs, devices and biologics on the manufacturer’s intended use.
Defining relevant characteristics in terms of “the characteristics of specific cell type(s) and/or the matrix or other component(s) in the donor tissue that are involved in, and/or relevant to the manufacturer’s intended use in the patient” promotes patient safety by insisting on a reasonable and scientifically supportable rationale for using an HCT/P for a particular mechanism of action. This clarification balances the FDA’s dual responsibilities of protecting patients from undue safety risks while promoting the ongoing availability and continued development of HCT/P therapies.
Example of Non-Homologous Use: Decellularized adipose matrix used to accomplish the manufacturer’s intended use of a particular metabolic or systemic effect in the patient (e.g., reducing insulin levels in a diabetic patient) is non-homologous because decellularized matrix is not relevant to metabolic or systemic activity.
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Recommendation #2 – Provider-Based Risks: To reduce provider-created risks, the FDA should target provider behavior by collaborating with IFATS and comparable organizations to draw on, and supplement existing federal and state methods of certification, registration, and similar measures.
For a risk-reduction strategy to succeed, it must target the root cause of the risk. Revising, retracting or replacing interpretations of regulatory terminology can target the risks of cells behaving in an unsafe ways, but can do little to prevent providers from behaving in unsafe ways. Because the risks of irresponsible providers offering unsafe treatments are not exclusive to HCT/P therapies, many federal and state mechanisms already exist for identifying, disciplining and prohibiting clinics and clinicians from endangering patients.
IFATS shares the FDA’s concern about provider-related risks in the HCT/P sector and shares its determination to end or minimize these risks. IFATS respectfully requests the FDA to collaborate with it and comparable organizations to identify and draw on existing federal and state methods for curtailing provider misconduct, and developing additional protections in the form of provider certification, registration, monitoring and similar measures. At present, the §§ 351-361 regulatory framework does not – and cannot – adequately respond to this form of risk. Collaboration among stakeholders and coordination with existing means of provider oversight offers the most effective and efficient strategy for protecting patients from provider-created risk.
Therefore, IFATS respectfully requests the FDA to meet with IFATS, the American Association of Blood Banks and other accreditation bodies for the purpose of working together to identify provider-focused safety objectives and measures that can be translated into formal accreditation requirements and interpretive guidance.
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Recommendation #3: Recognize that adipose HCT/Ps have both structural and nonstructural functions, and regulate based on its manufacturer’s intended use in the patient.
IFATS requests the FDA to expand its definition of adipose tissue from exclusively structural in function to include both structural and/or nonstructural functions, depending on the manufacturer’s intended use in the patient. This modification is critically necessary in order to:
- Reconcile the interpretive guidance on the definition and regulation of adipose with applicable statutory and regulatory requirements;
- Reflect and ensure biological accuracy; and most importantly,
- Regulate an HCT/P’s risks based on the manufacturer’s intended use and mechanisms of action in the patient.
a. Recognizing adipose tissue’s structural and/or nonstructural functions is required by applicable statutory and regulatory requirements.
Adipose HCT/Ps must be defined as having structural and/or nonstructural functions to align the draft guidance with statutory and regulatory recognition that cells and tissues may have more than one function. According to 42 USC § 321(g)(1), “[t]he term ‘drug’ means … (B) articles intended for use in the diagnosis, cure, mitigation, treatment, or prevention of disease in man or other animals; and (C) articles (other than food) intended to affect the structure or any function of the body of man or other animals; and (D) articles intended for use as a component of any article specified in clause (A), (B), or (C). (emphasis added). Statutory directives to focus on intended use pervade FDA regulation, including the regulation of drugs, biologics, devices, cosmetics, pesticides and more. Applicable statutes and regulations explicitly and implicitly recognize that the human body is complex, and its tissues and cells are often versatile and multi-functional. For example, 21 CFR 1271.3(c)’s definition of homologous use correctly recognizes that an HCT/P may have more than one “basic function.” It never says or even suggests that an HCT/P can only have one function, or that the regulator has sole authority to define that function and thereby dictate a manufacturer’s intended use. And yet the draft guidances do just that by insisting that adipose HCT/Ps are solely structural.
To align interpretive guidance with the regulations and statutory provisions being interpreted, IFATS respectfully requests the FDA to avoid pre-determining specific functions and uses for specific HCT/Ps. Instead, it should base regulations and guidance on the HCT/P’s function(s) and characteristic(s) that are relevant to its intended use by the manufacturer.
b. Recognizing adipose tissue’s structural and/or nonstructural functions is necessary to correct factual inaccuracy.
Regulation 21 CFR 1271.10(a)(4) categorizes an HCT/P as “either” structural or nonstructural, depending on its function. A structural HCT/P “does not have a systemic effect and is not dependent upon the metabolic activity of living cells for its primary function.” A nonstructural HCT/P “has a systemic effect or is dependent upon the metabolic activity of living cells for its primary function.”
The draft adipose guidance expressly acknowledges that adipose tissue contains adipocytes, preadipocytes, fibroblasts, vascular endothelial cells, a variety of immune cells, and also stores energy in the form of lipids. Citing only Junqueira ’s Basic Histology: Text & Atlas , the draft guidance classifies adipose as a connective and therefore structural tissue. This result is internally inconsistent and factually inaccurate – and the FDA’s sole cited authority explains why.
Junqueira classifies connective tissue as: 1) connective tissue proper; 2) embryonic connective tissues; and 3) specialized connective tissues. The latter category defines specialized connective based on their principal specialized functions. Blood, reticular connective tissue, adipose tissue, bone and cartilage all qualify as specialized connective tissues with specialized, nonstructural functions. Junqueira ’s examples include the following:
- Blood is a specialized connective tissue; its principal function of transport is nonstructural.
- Reticular connective tissues include the liver, pancreas, bone marrow and lymph They are nonstructural tissues because their principal functions are metabolic, including endocrine.
- According to Junqueira – the FDA’s sole cited authority – adipose tissue is nonstructural specialized connective tissue; its primary function is metabolic with co-existing structural
Junqueira ’s categorization of adipose as primarily nonstructural reflects longstanding scientific consensus. In1893, Gustav Neuber described his use of fat grafting in the orbital region to heal the adherent scarring which was the sequela of osteomyelitis. As a result of its nonstructural healing functions, the fat graft transformed facial scarring to more normal appearing skin and subcutaneous tissues.  In 1912, Holländer described the successful use of fat injections to prevent the recurrence of scarring following breast surgery.  In 1926, Charles Conrad Miller developed a new system for injecting fat grafts, and described 36 cases of correcting cicatricial contraction on the face and neck, and reported “excellent results” for another 2 cases after using fat grafts to treat “very persistent parotid fistulas…which defied all other methods of treatment.”  These and similarly favorable outcomes resulted from fat’s transformational nonstructural repair of the tissues into which it was placed. 
The understanding of the diverse roles of adipose tissue has steadily expanded , due in large part to the discovery of the first widely accepted adipokine, leptin, in the mid-1990’s.  Adipose tissue secretes proteins with systemic actions on hematopoietic, reproductive, metabolic, and other cells and tissues demonstrates unequivocally that adipose meets the definition of a true “endocrine” organ.[11,12] A Google scholar search of all available online medical and research databases for “the primary function of Adipose Tissue” returns 538,000 journal articles. Although the search did not designate a specific function, the search results referred to adipose tissue almost exclusively as a nonstructural metabolic and endocrine organ with secretory properties. A search for an exact match of the phrase “primary function of adipose tissue” yielded the following: “It was long believed the primary function of adipose tissue was energy storage; in fact, stromal adipose is a complicated endocrine organ.” However, even energy storage is nonstructural.
The FDA’s draft guidance on minimal manipulation defines nonstructural tissues as “serv[ing] predominantly metabolic or other biochemical roles in the body such as hematopoietic, immune, and endocrine functions.” To illustrate, the draft guidance offers “cord blood, lymph nodes, pancreatic tissue” as examples of nonstructural tissue. These tissues are indeed nonstructural – but they are also specialized connective tissue, as explained in Junqueira. In addition adipose has “hematopoietic, immune, and endocrine functions,” as explained below. As demonstrated by Junqueira, adipose HCT/Ps clearly do more than “reconstruction, repair, or replacement that relate to its utility to cushion and support the other tissues in the subcutaneous layer (subcutaneum) and skin.” And the FDA’s own nonstructural examples prove that classifying connective tissue, including adipose tissue as solely structural is factually inaccurate and logically flawed.
Thus, IFATS strongly recommends that the draft guidances be revised to define and categorize adipose tissue has both structural and nonstructural functions.
In support, IFATS offers the following examples of adipose’s nonstructural, and combined nonstructural and structural functions.
Nonstructural Functions of Adipose HCT/Ps:
- Nonstructural Endocrine Functions – It is well recognized that adipose is an endocrine organ which, like other endocrine organs, performs a variety of nonstructural Adipose tissue secretes proteins with nonstructural, systemic actions on hematopoietic, reproductive, metabolic, and other cells and tissues. [11, 12]
- Glucose and lipid metabolism control via adipokine secretion 
- Reproductive and endocrine control via adipokine secretion [14-16]
- Immunomodulatory and immunosuppressive systemic control via cytokine and protein factor secretion [17-22]
2. Nonstructural Paracrine Functions
- Angiogenic control via vasculogenic cytokine secretion [22-26]
- Hematopoietic control via cytokine secretion, both locally and systemically 
- Neurogenesis via secretion of cytokine factors [28-34]
3. Nonstructural Hematopietic Potential of adipose stem cells in adipose deposits
- Reservoir for hematopoietic and lymphoid progenitor cells similar to bone marrow [18, 35, 36]
- Thermogenesis (brown and beige fat)[37-41]
- Energy reservoir (white adipose depots) [42,43]
4. Nonstructural Promotion of Lactation
- Fat serves as an energy reservoir and nutrient supply for breast epithelial cells.
- As pregnancy progresses, the breast epithelium proliferates in a branching manner to occupy the majority of the adjacent adipose tissue and
- At parturition, the epithelial cells draw on the lipid reserves of adipocytes within immediate proximity and secrete these nutrients into the milk available to the newborn infant during
- As long as the mother continues to breast feed the infant, the epithelial cells remain viable and
- If suckling is discontinued for periods of 24 to 48 hours, the epithelial cells undergo rapid apoptosis, leaving pre-adipocytes and adipocytes as the predominant cell within the breast
- While the presence and organization of epithelial cells within the breast tissue provide it with a unique architecture, the mammary adipocytes themselves show remarkable similarity to adipocytes from elsewhere in the Thus, the mammary fat pad displays homology to other adipose tissue depots.
5. Nonstructural Regenerative Functions
- Local and circulating multipotent progenitor cells can repair and regenerate damaged tissues such as repairing irradiated skin, alleviating fibrotic changes, improving mobility and vitality, and repairing structures such as hair follicles and [45-47]. Specific examples include:
- Modulation of scarring
- Treatingold burn scars [55-57]
- Release of adherent scarring/fasciotomies 
- Modulation of scarring in primary cleft lip repair 
- Multipotent progenitor cells may be recruited for repair and regeneration of ischemic damage induced by acute myocardial infarction. 
- Adipose mesenchymal stem cells as progenitor cells in a perivascular position contribute to vascular network formation and vascular structures.[49-52] As such, the adipose mesenchymal stem cells are located in a position and serve a role shared by mesenchymal stem cells located in nearly all body tissues . Adipose MSCs located in a range of tissues can enhance vascularity and perfusion, and thus provide cells that are precisely homologous to those already present in the
- Adipose mesenchymal stem cells induce a monocyte/macrophage phenotype switch from M1 to M2 macrophages, contributing to improved infarct healing post-acute myocardial 
6. Nonstructural Functions in Bone Marrow – Bone marrow and blood products are exempt from regulation under § 351 and 361. For over 40 years, it has been clearly established that adipose is present in bone marrow where it serves a wide variety of nonstructural functions. The following physiologic processes have nothing to do with providing cushioning and support and therefore are not properly categorized as a structural use or function of adipose cells. 
- Pre-adipocytes as mesenchymal cells in bone marrow: Bone marrow contains a spectrum of mesenchymal cells, including pre-adipocytes that can perform the nonstructural function of differentiating into adipocytes, osteoblasts and chondrocytes depending on the organism’s current needs.
- Pre-adipocytes and adipocytes regulate lympho-hematopoiesis and enable the bone marrow microenvironment to regulate proliferation within blood cell lineages to favor erythropoiesis rather than Adipocytes also contain nonstructural metabolic precursors and energy for the purpose of lympho-hematopoiesis. This is a nonstructural function.
- Adipocytes are essential for synthesizing plasma membranes during blood cell development because they contain cholesterol esters, triglycerides and lipoproteins.
- Bone marrow and extramedullary adipocytes are critical for homeostatic control of temperature in the bone marrow microenvironment and throughout the body, and thus contribute to the overall energy metabolism of the organism.
- Bone marrow adipose tissue is an essential endocrine organ. Bone marrow adipose tissue (MAT) increases during caloric restriction (CR), is responsible for increased adipokine secretion, and alters skeletal muscle adaptation to These and other observations identify MAT as an endocrine organ.
BOTH Nonstructural and Structural Functions: In the following examples, adipose’s structural and nonstructural functions combine for the patient’s benefit:
- Reversal of damage caused by therapeutic radiation [60-63]
- Structural (filling tissue defect) uses, and
- Nonstructural tissue repair and regenerative uses 
- Treatingacute thermal injury [64-65]
- Treating Pain Mitigating implant breast pain 
- Improving post-mastectomy pain [67-68]
- Improving lower back pain 
- Nerveor neuroma repair [71-72]
- Healing ulcers
- Treatingpressure sores 
- Treating chronic non-healing anal fissures and associated stenosis 
- Treating vocal fold paralysis [75-77]
- Treating velopharyngeal insufficiency 
- Treating scleroderma and systemic sclerosis 
- TreatingDupuytren’s disease of the hand [80, 81]
- Treating Raynaud’s phenomenon – After fat grafting, there is improved symptomatology with evidence suggestive of measurably increased perfusion 
- Improving tendon repair
- Adipose tissue assists in tenolysis for foot and hand tendon 
- Treating adherent tendons and joints in burn patients with fat graft 
- Preventing osseous reunion of skull defects 
- Improvingthe quality of skin 
c. Regulating an HCT/P’s risks based on the manufacturer’s intended use and mechanisms of action in the patient ensures meaningful evaluation and effective regulation of risk.
The §§ 351-361 framework conditions the degree of regulatory oversight on the degree of an HCT/P’s risk. The homologous use and minimal manipulation criteria are central to determining whether an HCT/P will be classified as a § 361 or § 351 product, and if the latter, whether § 351’s “same surgical procedure” exception will apply. In turn, the existence of homologous use and minimal manipulation depend on the HCT/P’s structural or nonstructural function. More specifically:
21 CFR 1271.3(c) defines homologous use as “the repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues with an HCT/P that performs the same basic function or functions in the recipient as in the donor.”
21 CFR 1271.3(f) evaluates minimal manipulation of structural tissue in terms of processing that does not alter “the original relevant characteristics of the tissue relating to” the tissue’s utility for reconstruction, repair, or replacement. For nonstructural tissues, it evaluates “the relevant biological characteristics of cells or tissues.”
Insisting that adipose be evaluated as exclusively structural precludes any evaluation of its nonstructural functions despite their presence and importance in the donor and intended use and therapeutic benefits for the recipient. Failing § 361’s homologous use and minimal manipulation criteria are virtually guaranteed. This effectively prohibits any nonstructural use, and precludes any meaningful evaluation of their risks.
As a result, it effectively prohibits patient access to safe nonstructural applications of adipose tissue and thereby undermines the FDA’s obligations to protect patients and promote innovation.
The “ same surgical procedure” exception to § 351 also becomes completely unavailable for nonstructural use of adipose because it similarly requires homologous use and minimal manipulation.
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Recommendation #4: Revise the evaluation of minimal manipulation and homologous use as they pertain to particular applications of adipose tissue, (as detailed below).
IFATS respectfully requests the FDA to reconsider three particular applications of adipose tissue with regard to homologous use and minimal manipulation, each of which is required for § 361 classification as well as § 351’s “same surgical procedure” exception. In specific, IFATS requests the FDA to change its prior examples of the absence of homologous use and/or minimal manipulation to recognize the following:
- Decellularizing adipose tissue for structural use is minimal manipulation.
- Structural use of fat in the breast constitutes homologous
- Stromal vascular fractionation (SVF) of adipose to obtain nonstructural adipose components for use as a nonstructural tissue constitutes minimal
Each is explained in order
a. Decellularizing adipose tissue for structural use is minimal manipulation.
The draft guidance currently states that decellularizing structural adipose tissue constitutes more than minimal manipulation because the process alters the tissue’s ability to perform structural functions. This is incorrect. Adipose tissue’s structural functions are performed by a dense and interconnected skeleton of reticular fiber and dense connective tissue. Its biomechanical properties include tensile strength and elasticity, both of which are central to the structural functions of padding and cushioning.
Nonstructural components such as adipocytes, pre-adipocytes, lipids, etc. do not contribute to adipose’s structural characteristics or functions. It is well recognized that decellularization leaves adipose’s structural components fully intact. It does not alter, disturb or weaken the remaining reticular fiber and dense connective tissue skeleton, or compromise its ability to perform structural functions. Multiple reports have demonstrated that decellularized adipose tissue retains structural properties and can be injected to impart padding and cushioning of soft tissues. [89-93]
The FDA already classifies decellularized dermis as minimally manipulated, thereby acknowledging that the process of decellularization does not alter structural characteristics or functions of the remaining structural matrix. Removing cells from dermis and removing cells from adipose employ comparable methods to achieve comparable results. Decellularizing adipose for structural use, like decellularizing dermis for structural use, does not alter structural characteristics.
For these reasons, IFATS respectfully requests the FDA to revise the draft guidance to recognize that decellularized adipose is minimally manipulated as required by § 361 and § 351’s “same surgical procedure exception.”
b. Structural use of fat in the breast constitutes homologous use.
Example B-3 of the draft adipose guidance states that application of adipose-based HCT/Ps to the breast is nonhomologous use because “[t]he basic function of breast tissue is to produce milk (lactation) after childbirth. Because this is not a basic function of adipose tissue, using HCT/Ps from adipose tissues for breast augmentation would generally be considered a non-homologous use.” This logic is flawed and must be corrected because it mischaracterizes the function of the breast, and mischaracterizes the function of adipose in breast surgery.
- For the purpose of determining homologous use, the basic function of the breast is a secondary sex organ. In terms of shape, form and appearance, the breast is vital to a woman’s bodily integrity and body image, psychological sense of self, and overall physical and emotional health and well-being.
- Lactation is not the sole or even primary function of the breast.
- Most women never lactate, but their breasts do function as secondary sex organs throughout their adolescence and
- When lactation does occur, it is episodic, time-limited, and accounts for a very small fraction of a woman’s lifespan.
- Even when healthy, post-menopausal women cannot Restoring lactation is thus completely irrelevant to restoring breast function.
- All men have breasts, thousands develop breast cancer each year, and many will need reconstructive surgery — even though men do not lactate.
- Federal law recognizes and protects the breast’s importance as a secondary organ.
- The Women’s Health and Cancer Rights Act, 29 USC 1185b(a), requires group health insurers to cover “all stages” of breast reconstruction following mastectomy or irradiation, including bilateral correction of asymmetrical appearance where one breast is otherwise unaffected.
- Restoring lactation is not a goal or even a remote concern of this In fact, lactation is never mentioned in the statute’s text, legislative history or associated regulations.
- The function of adipose tissue in breast surgery is structural and therefore
- Mastectomy removes more than the ability to lactate. It removes size, shape and form by removing the breast mound, which is predominantly adipose. Consequently, applying adipose tissue for the structural purpose of restoring form and shape is homologous use.
- By classifying adipose based tissues as non-homologous when applied to the breast, an entire class of Centers for Medicare & Medicaid Services (CMS) approved breast reconstruction procedures would be at risk for not complying with the same surgical procedure For example:
- Autologous free tissue flap transfer (“free flap” breast reconstruction) is performed by transferring complex musculocutaneous flaps containing adipose One of the most common methods of reconstruction, it qualifies as an HCT/P because it completely removes fat-containing tissue flaps from the body before implanting. [94-96] Fat grafting for breast reconstruction is another common clinical practice.
- According to the draft adipose guidance, these and other methods of breast reconstruction could no longer be used without formal premarket approval because they do not restore lactation and are therefore non-homologous. Focusing solely on restoration of lactation ignores the fact that the breast is largely composed of fat tissue and its size, shape and form can be reconstructed with fat
- This and other methods of breast reconstruction will no longer be available for clinical use under 361 or § 351’s same surgical procedure exception because they will not restore lactation.
- Removing these and other reconstructive methods from clinical application has nothing to do with risk. It is instead a perverse outcome of insisting that breast reconstruction be evaluated for its ability to restore the breast’s minor and episodic function of lactation despite fat’s ability to restore the breast’s size, shape and function as a secondary sex organ.
For these reasons, IFATS respectfully requests the FDA to revise the draft HCT/P guidance documents to recognize that as applied to the breast, adipose tissue is homologous use because it performs the structural functions of restoring, repairing or reforming size, form and shape.
c. When intended for a nonstructural use in the patient, stromal vascular fractio n (SVF)cells should be evaluated as nonstructural when determining minimal manipulated and homologous use.
The FDA’s draft adipose guidance expressly acknowledges that adipose tissue contains a variety of nonstructural components, including adipocytes, preadipocytes, fibroblasts, vascular endothelial cells, a variety of immune cells, and also stores energy in the form of lipids. These are nonstructural because the cells perform the same regenerative functions in vivo as they do in vitro and animal models. [97- 98] Nonstructural adipose HCT/Ps are readily available in the stromal vascular fraction (SVF). Stromal vascular fractionation of lipoaspirate (typically obtained through liposuction) can remove fat’s structural components, making nonstructural SVF cells available for nonstructural use in a patient. Just as removing nonstructural cells through decellularization does not alter the relevant structural characteristics or structural function of the remaining structural matrix, removing structural components does not alter the relevant nonstructural characteristics or nonstructural function of the remaining nonstructural SVF components.
This is minimal manipulation under 21 CFR 1271.3(f)(2) because extracting nonstructural cells or tissues from lipoaspirate “does not alter the relevant biological characteristics of cells or tissues.”
Also, this is homologous use under 21 CFR 1271.3(c) because it uses lipoaspirate’s nonstructural HCT/Ps for “repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues with an HCT/P that performs the same basic function or functions in the recipient as in the donor.”
Examples: Nonstructural adipose tissue for homologous use, with minimal and more than minimal manipulation à Reversal of radiation damage as intended nonstructural use
Homologous use with no manipulation: Using liposuction aspirate to perform fat grafting/adipose tissue therapy for the intended use or of reversing radiation damage in the breast – a nonstructural function – is homologous use. The structural side-effect of increasing volume may be a collateral benefit, but the intended use is still nonstructural tissue repair.
- Use is homologous because the HCT/P performs that same basic nonstructural function in both donor and
Homologous use with minimal manipulation: Using liposuction aspirate is indicated for the nonstructural function of reversing radiation damage in the neck without the volume gain of a fat graft. Separating nonstructural from structural components obtains nonstructural SVF cells for nonstructural use in the patient.
- Use is homologous because it is performing the intended nonstructural function of reversing radiation
- Manipulation is minimal because processing does not alter relevant nonstructural biological characteristics.
Homologous use with more than minimal manipulation: Using liposuction aspirate is indicated for the nonstructural function of reversing radiation damage in the intestines by catheter injection of nonstructural SVF. However, an adequate dose is difficult to obtain because the patient is cachectic (low body fat caused by caloric depletion from radiation enteritis). Culture expansion is considered as a means of increasing dose.
- Use is again homologous because SVF cells would perform the intended nonstructural function of reversing radiation
- Manipulation is more than minimal because culture expansion of cells to yield a therapeutic dose alters relevant biological characteristic. SVF cells in their natural state do not engage in linear growth to create a homogeneous monoculture. Even tumors do not produce homogeneous monocultures.
Examples: SVF for nonhomologous use >>>Bone (re)generation
SVF cells do not normally form bone in their native location. Delivering SVF cells to bone for the intended structural function of directly (re)generating new bone (via action of “stem cells”) might be considered. Processing might involve combining SVF cells with one or more additives (such as ex vivo culture media additives) for the intended structural function of (re)generating NEW bone (such as additives added to our culture medias ex vivo). For this scenario:
- Use is nonhomologous because the basic function in donor and patient will differ if nonstructural SVF cells are combined with one or more additives (such as ex vivo culture media additives) for the intended structural function of (re)generating NEW bone (such as additives added to our culture medias ex vivo).
- Manipulation is more than minimal because processing would alter the nonstructural SVF’s original relevant characteristics.
◊ ◊ ◊ ◊ ◊ ◊ ◊
The members of IFATS are grateful for the FDA’s willingness to re-open and extend the period for public comments and allow additional time for the September 2016 public hearing on the 2014-2015 draft HCT/P draft guidances. As a multidisciplinary scientific society composed of adipose stem cell biologists and clinician–scientists, IFATS would greatly appreciate the opportunity to work with the FDA in meeting the challenges of regulating HCT/P therapies. We respectfully request that representatives of the FDA, including the Director of CBER, meet with members of IFATS to discuss the issues addressed herein as well as others that pertain to the advancement and regulation of adipose-based therapies.
Respectfully submitted on behalf of IFATS,
Adam J. Katz, MD, FACS
Chair, IFATS Regulatory Affairs Committee & IFATS Co-Founder University of Florida College of Medicine
Director of Plastic Surgery Research,
Laboratory of BioInnovation and Translational Therapeutics Division of Plastic Surgery, Department of Surgery
IFATS BOARD OF DIRECTORS
Bruce Bunnell, PhD
Tulane University / United States
Louis Casteilla, PhD
University of Toulouse / France
Sydney Coleman, MD
New York & Pittsburgh Universities / United States
Julie Fradette, PhD
Lavalle University / Canada
William Futrell, MD
Founders’ Board University of Pittsburgh / United States
Marco Helder, PhD
VU University Medical Center Amsterdam / The Netherlands
Adam J. Katz, MD, FACS
Founders’ Board University of Florida / United States
Ramon Llull, MD, PhD –
Founders’ Board University of Barcelona / Spain
Kacey Marra, PhD
University of Pittsburgh / United States
Ricardo Rodriguez, MD –
President (2016) Private Practice / Johns Hopkins / United States
Peter Rubin, MD, FACS – Chair, Founders’ Board Chairman of the Board
University of Pittsburgh / United States
Stuart K. Williams, PhD
University of Louisville / United States
Jeff Gimble, MD, PhD
Pennington Biomedical / United States
Keith March, MD, PhD
Indiana University / United States
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After receiving a personal invitation from President Susumu Takayangi MD, Global Stem Cells Group and its Korean biotechnology partner, N-Biotek, participated in the 23rd Congress of the International Society of Aesthetic Plastic Surgeons that took place this October in Kyoto. With the assistance of more than 1500 physicians and surgeons from around the globe, the conference’s focus was to grow a global understanding of the importance of the aesthetic modalities in plastic and reconstructive surgery and the applications of cellular and regenerative medicine in this field.
The Congress brought together an expert panel for a series of lectures and discussions that hope to provide solutions and create new techniques in the coming years to be applied in plastic and reconstructive surgery. Influential scientific and clinical experts stood together and evaluated the state of research and clinical application.
The keynote speaker in the conference was the Nobel Laureate Shinja Yamanaka. Dr Yamanaka won the award on 2012 for his creation of induced pluripotential stem cells. Yamanaka addressed among other things the importance of the regenerative medicine field opening a new frontier for the treatment of chronic degenerative conditions.
“We are excited to have attended the ISAPS conference once more. Our goal, as always, is to promote scientific training for physicians and research in stem cell technology that we hope will contribute to the global discussion on how these therapies can advance human health and longevity” says Benito Novas, founder and CEO.
Mr. Sanguk Ra, Business Director of N-Biotek, a biotechnology added ;”these are very exciting times, we see more and more physicians getting involved in regenerative medicine, and that of course puts more pressure on the scientists to help advance the field”
Both companies, as leaders of the industry, launched their new Turnkey Solution, a compact and cohesive laboratory as a concept for physicians’ offices, and the new Progenikine Kit, an enzyme based closed system for isolation of adipose derived stem cells. To learn more, visit the Global Stem Cells Group website, www.stemcellsgroup.com.
About Global Stem Cells Group:
Global Stem Cells Group, Inc. is the parent company of six wholly owned operating companies dedicated entirely to stem cell research, training, products and solutions. Founded in 2012, the company combines dedicated researchers, physician and patient educators and solution providers with the shared goal of meeting the growing worldwide need for leading edge stem cell treatments and solutions. With a singular focus on this exciting new area of medical research, Global Stem Cells Group and its subsidiaries are uniquely positioned to become global leaders in cellular medicine. Global Stem Cells Group’s corporate mission is to make the promise of stem cell medicine a reality for patients around the world. With each of GSCG’s six operating companies focused on a separate research-based mission, the result is a global network of state-of-the-art stem cell treatments.
Global Stem Cells Group has successfully hosted its 3rd Annual International Symposium on Stem Cells and Regenerative Medicine.
On the 28th of September 2016, Global Stem Cells Group successfully hosted its 3rd annual International Stem Cells and Regenerative medicine symposium in Buenos Aires Argentina. This International Stem Cells and Regenerative medicine symposium was dignified by the participation of the most renowned and experienced physicians on stem cell and regenerative medicine from Latin America and Europe.
The symposium was also graced by an online participation of several physicians through online streaming and a speaker lineup which included highly coveted personalities from the areas of scientific research, clinical research and regulatory world. This team of prominent and talented specialists in medical research and international stem cell used the forum to share the recent discoveries and advancements in scientific research and medical science.
During the inauguration of the symposium, Global Stem Cells Group honored Dr. Joseph Purita for his lifetime achievements and contributions to the cellular and regenerative medicine and his guide to the Global Stem Cells Group faculty from its inception. Dr. Purita is a world renowned orthopedic surgeon and the pioneer in laser techniques in orthopedic surgery and the application of stem cells and PRP therapies for orthopedic injuries and diseases.
After the symposium, Stem Cell Training the group’s arm for physician education hosted a full enrollment workshop where several physicians from Latin America and Europe received hands on training on isolation and reintegration of adult stem cells, use of peptides and cellular secretions and orthomolecular interventions. The physicians were trained on global skills and capabilities required for the treatment of patients in the course of their medical practices and for career development.
To learn more about the 3rd Annual International Symposium on Stem Cells and Regenerative Medicine, visit the Global Stem Cells Group website, or email email@example.com, or call 305-560-5337.
About Global Stem Cells Group:
Global Stem Cells Group, Inc. is the parent company of six wholly owned operating companies dedicated entirely to stem cell research, training, products and solutions. Founded in 2012, the company combines dedicated researchers, physician and patient educators and solution providers with the shared goal of meeting the growing worldwide need for leading edge stem cell treatments and solutions. With a singular focus on this exciting new area of medical research, Global Stem Cells Group and its subsidiaries are uniquely positioned to become global leaders in cellular medicine.
Global Stem Cells Group is proud to present our 2016 Edition of our Regenerative Medicine Symposium, to be held in 6 different Cities around the World.
This prestigious event will have the presence of a select group of renowned international speakers who will offer a combined day of conferences of high scientific rigor aimed at Physicians.
Global Stem Cells Group’s symposium will provide cutting-edge information on developments in all areas of stem cell research, including the biology, medicine, applications, regulations, product development, and the commercialization of stem cells.
Business opportunities, challenges, and potential strategies for overcoming these challenges will also be addressed. Come join us to learn what categories of companies are currently commercially viable, how they’re being funded, and what kind of strategic relationships are available within the industry
Our First Event of the Year will Take Place in Bogota Colombia in March 3 rd for more info and registration click here http://stemcellconference.org/es/simposio-bogota
Global Stem Cells Group offers a stem cell training course that can help you bring some of our cutting edge regenerative therapies to your practice or clinic. We offer an intensive, hands-on two day training class, show you how to collect fat tissue via our precision mini lipo-aspiration technique, and we walk your through the process of collecting bone marrow via the iliac crest.
We provide didactic lectures on stem cell structure, function and treatment, and we go through the techniques to isolate and harvest stem cells from fat tissue and bone marrow, as well as the platelet rich plasma from the peripheral blood. We do all of this on actual patients! Three to four of them during the two-day course period, so that you can get a good understanding on how to perform the different techniques and procedures.
GSCG will provide you with written protocols, forms and consent so that you can easily implement this in your practice after certification. We also offer instructional videos, quality control (QC) assays, viability and cell counts. Our courses are fully accredited— providing 16 categories for one credit—by three different universities, and we offer the opportunity to participate in Institutional Review Board (IRB) clinical studies.
Now, you’re probably wondering about our products, without which you’d have the weekend off.
For the bone marrow product, we’ve partnered with a company called Emcyte. All of the kits for the bone marrow and the platelet rich plasma are FDA 510K approved, thus allowing for the collection of a small sample of blood, which can be safely attained, to produce concentrated growth factors and platelets. The system is gentle and processes bone marrow aspirate precisely for the purest concentration of cells at the point of care.
Also, there’s our —adipose-derived stem cell Kit . fact: fat tissue is one of the most plentiful sources of stem cells in the body—in particular, the mesenchymal type stem cells. Imagine, you can get about five hundred times more mesenchymal stem cells from fat tissue than you can get from the bone marrow. Fat tissue is a perfect source of stem cells when you’re treating degenerative type diseases, or replenishing some of the tissue that has been damaged as a result of injury and or degenerative disease.
Within this population there exists a multipotential progenitor cell that has the ability to go down the adipogenesis pathway, the chondrogenesis and the osteogenic pathway. These cells are very angiogenic and vasculogenic in nature, meaning they are able to form blood vessels and are very useful in ischemia type conditions.
Global Stem Cells Group has a variety of isolation kits, and all of our kits are produced according to good manufacturing processes as I mentioned before. We have a full scale laboratory in Santiago Chile where we produce all of the reagents that are necessary, and our kits include all of the consumables necessary to isolate regenerative stem cells. You can visit the Adimarket.net website to view the variety of different products we offer, all of which can assist with some of the work you are doing in-clinic. Including things like centrifuges and other medical devices and equipment that are necessary to bring stem cell therapies to the patients. We also go over this information during the stem cell training course and teach you exactly what equipment you will need to perform some of the techniques.
Stem cell therapies may hold the cure to Alzheimer’s, although so far that cure has been elusive. People who suffer from Alzheimer’s disease experience disorientation regarding time and place, changes in mood, personality and behavior, memory loss, difficulty solving problems or planning, and difficulty writing or performing other routine and familiar tasks. This progressive and irreversible brain disorder may affect judgment, initiative and social life, and can lead to physical symptoms such as vision problems.
Alzheimer’s affects mostly people aged 70 years and above, and is more common in women. It is the main risk factor for dementia among the elderly.
There is no known cure for Alzheimer’s. Conventional treatments, both drug-based and non-drug strategies, may help with cognitive and behavioral symptoms, but have little to no effect on the disease’s development over the long term. Current medications can’t stop Alzheimer’s from progressing, but they can temporary lessen symptoms like confusion and memory loss.
Although there have been attempts to find a remedy for Alzheimer’s, and despite the fact that scientists have managed to effectively treat lab animals with drug-based treatments, no animal model has managed to truly mimic its symptoms as they manifest in humans. Remedies that worked in lab animals have failed to work in humans; for this reason, scientists decided to try a different approach by exploring the possibilities of stem cells therapies in Alzheimer’s treatments.
Can Stem cells develop new Alzheimer’s treatments?
Alzheimer’s disease affects neurons in all parts of the brain, and the complexity of this condition makes it difficult to create a model that perfectly mimics its manifestations. At least in theory, stem cells could be used for treating this condition by transplanting neural stem cells into the patient’s brain in an attempt to generate healthy new neurons to replace dead and damaged neurons. It remains unclear whether the brain would be able to integrate the transplanted cells, and if the neural stem cells are able to travel to the damaged areas.
Another great challenge is producing the different types of neurons needed to replace the damaged cells, and to find a way to stimulate the renewal of the lost connections between neural cells. Even if the transplanted cells survive and find their way to the damaged areas, they might become damaged by proteins that build up in the brain—the same proteins that cause the disease in the first place, which means any effects of a stem cell transplant could be only temporary.
A different approach would be to use stem cells for delivering neurotrophins to the brain. Neurotrophin proteins support the growth and survival of neurons, but in patients with Alzheimer’s, they’re produced in amounts too low to contribute such support. Neural stem cells can produce such cells, and in mice tests this method did prove helpful; scientists observed some improvements in memory in mice treated with stem cells.
Mesenchymal stem cells could also be used for treating Alzheimer’s—not to replace damaged neurons, but to heal them. Mesenchymal stem cells may exert anti-inflammatory effects and may help ameliorate the symptoms of Alzheimer’s, but there’s no study at the moment to prove their safety or effectiveness in this condition .
Although clinical trials and studies on Alzheimer’s disease treatment to date have a high failure rate, the use of stem cells may be helpful for studying the behavior of brain cells damaged by the condition, as well as for testing various therapeutic approaches and predicting which treatment may help Alzheimer’s patients.
Researchers from the Harvard Stem Cells Institute took skin cells from Alzheimer’s patients and reprogramed them to create induced pluripotent stem cells (iPSC); obtained cells were grown in special lab conditions and were found to release the same proteins that form plaque in Alzheimer’s patients . This may give scientists the opportunity to study the behavior of Alzheimer’s-affected cells and to search for and test new remedies.
Asian scientists managed to turn human fibroblasts into neuronal cells using a chemical cocktail of small molecules . These findings may provide an alternative strategy for modeling the neurodegenerative disorder, which may help scientists understand the mechanisms behind this condition. It may also play an important role in identifying new stem cell based treatments.
Healing damaged lungs with stem cells.
A New study published by scientists from the Weizmann Institute of Science suggests that stem cells may be used for repairing damaged lung tissue. This discovery gives new hope for treating conditions like bronchitis, asthma, cystic fibrosis or emphysema, which affect more than 35 million Americans and are the second leading cause of death worldwide.
Bone Marrow stem cells able to generate new lung tissue
The treatment method proposed by scientists at the Weizmann Institute is based on the similarities between stem cells that reside in the lungs and those in bone marrow. Bone marrow stem cells, when transplanted to a patient, manage to find their way through the blood and to navigate to the designated area where they differentiate.
Acknowledging the similarities between lung and bone marrow stem cells, Professor Yair Reisner of the Immunology Department of the Weizmann Institute tested the ability of lung stem cells to travel to a specific region after transplantation in mice . Before introducing the bone marrow stem cells into mouse models with lung damage, the group of scientists cleared the lungs’ stem cell compartments to clear a path for the transplanted cells.
The injected stem cells managed to reach the empty lung compartments and settle in the lungs, where they differentiated into normal lung tissue,six weeks after transplantation. Results showed that new lung cells continued to be created from the transplanted stem cells 16 weeks after the implantation, ultimately healing the damaged lungs and improving their breathing ability.
The Weizman scientists intend to continue their research by exploring this option further, and possibly create a bank of lung stem cells that can provide cells ready to be transplanted to patients with severe respiratory diseases.
Lung-specific induced pluripotent stem cells (iPSCs)— potential alternative to bone marrow stem cells
This was not the first attempt to heal damaged lungs with stem cell transplants. In a previous study, scientists from the Boston University Medical Center managed to generate 100 new lines of lung-disease specific iPSC from patients with emphysema, cystic fibrosis and other similar conditions. Results suggest that the new stem cell lines could be used for transplantation in patients suffering from lung diseases, thanks to their ability to differentiate to endoderm cells that give rise to lung tissue.
Darrell Kotton, the study’s lead author, highlighted the fact that iPSCs are easier to cultivate in lab conditions than bone marrow stem cells, and are genetically identical to the patient’s cells, so the risk of rejection in such transplants is eliminated. The lung-specific iPSCs obtained by manipulating adult stem cells into a primitive stem cell state could solve some of the hurdles impacting other kinds of stem cell research.
In this study, scientists used skin stem cells manipulated into primitive pluripotent stem cells, with results showing that the iPSCs have the ability to multiply and differentiate into endoderm tissue–the natural precursor of lung cells .
- Chava Rosen, Elias Shezen, Anna Aronovich, Yael Zlotnikov Klionsky et al. – Preconditioning allows engraftment of mouse and human embryonic lung cells, enabling lung repair in mice, Nature Medicine, 2015, http://www.nature.com/nm/journal/vaop/ncurrent/full/nm.3889.html
- Aba Somers, Jyh-Chang Jean, Cesar A. Sommer, Amel Omari et al. – Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette, Stem Cells, 2010, 28 (10):1728, http://onlinelibrary.wiley.com/doi/10.1002/stem.495/full
If you’re one of those people who is really fond of their beauty sleep, or who never compromises when it comes to getting their full eight hours per night, now you have one more reason to make a full night’s sleep a priority .
A study by scientists at the German Cancer Research Center have found that while environmental stress can damage the DNA in adult hematopoietic stem cells, a good night’s sleep can keep these cells young, contributing to a youthful appearance and preventing cancer.
Healthy sleep patterns lower the risk of DNA damage in stem cells
According to German researchers, under normal conditions a high number of different types of adult stem cells exists in a state of dormancy inside the human body, but they cannot divide, therefore cannot be used for tissue regeneration. This state of dormancy protects the stem cells from DNA damage, keeping us younger and preventing premature aging .
Yet, increased levels of stress in all its forms—from chronic infections to environmental stress—can trigger a rapid division of stem cells, kicked into gear as the body needs to repair its damaged tissues. In such conditions, the dormant stem cells go from no activity to very high activity in a short interval, and this rapid change forces them to increase their metabolic rate and synthesize new DNA.
Doctor Michael Milsom, who coordinated the German study, says that having to simultaneously execute such complicated functions increases the risk of DNA damage in the stem cells, reducing the ability of tissues to repair themselves and speeding up aging [1, 3].
Moreover, scientists believes that the accumulation of stress-induced damage in the stem cells can make one more prone to cancer. Experiments conducted in this study showed that cell division that takes place under stress leads to an increased production of reactive metabolites. These substances can damage DNA, causing the death of stem cells or leading to mutations that can contribute to cancer.
Understanding how to prevent the aging of stem cells or DNA mutations and damage could be the key to delaying the aging process and reducing the risk of developing certain forms of cancer, concludes Dr. Trumpp, co-author of the study’s research paper.
Protect your stem cells for healthy skin and a youthful appearance
The study is not the only one to prove a connection between sleep and the health of stem cells. Another paper, published in the journal of Cell Research by scientists from the University of California Irvine, showed that circadian rhythms regulate the metabolism of skin stem cells, and that getting enough sleep during the night can maintain healthy cell division, nurturing stem cell differentiation .
Although the study was conducted on mice, the findings are worth exploring further to determine whether a disruption in the healthy circadian rhythm can alter the normal function of stem cells, leading to accelerated aging.
Professors Andersen and Gratton, who conducted the Irvine study, focused on the effects stem cells have on the skin, already knowing that stem cells found in the dermal layers protect the skin and help in the repairing the epidermis after injuries.
Using innovative technologies, the two researchers measured the metabolic state of stem cells, discovering that the circadian clock does regulate one form of intermediary metabolism in target cells. According to researchers, it’s the same component of metabolism that creates oxygen radicals, harmful substances that can cause DNA damage.
The results of this study suggest that maintaining healthy sleep patterns can prevent DNA-damage in skin stem cells, while an altered internal clock could lead to the accumulation of damage in these cells, accelerating aging.